Directed bioconversion of Kraft lignin to polyhydroxyalkanoate by Cupriavidus basilensis B-8 without any pretreatment
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abstract
2016 This work presents here a new fundamental strategy for bio-converting Kraft lignin (KL) into useful products. Cupriavidus basilensis B-8 (here after B-8) was able to use KL as the sole carbon source. Fully 41.5% of lignin, 37.7% of total carbon (TC) and 43.0% of color were removed after 7days of incubation. At the same time, lignin was depolymerized into small fragments, which was confirmed by scanning electron microscopy (SEM) and gel permeation chromatography (GPC). Bacterial biomass accumulated to 735.6mg/L at the initial KL concentration of 5gL 1 , and the corresponding volumetric productivity of polyhydroxyalkanoate (PHA) was 128mg/L. PHA productivity was significantly improved through fed batch fermentation and reached to 319.4mg/L. GCMS analysis showed that PHA polymer was composed of three basic monomers: 98.3mol% of (S)-3-hydroxy-butanoic acid (S3HB), 1.3mol% of -3-hydroxybutyric acid (R3HB) and 0.4mol% of 3-hydroxy-butanoic acid (3HB).
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Process Biochemistry
author list (cited authors)
Shi, Y., Yan, X. u., Li, Q., Wang, X., liu, M., Xie, S., Chai, L., & Yuan, J.