TRIM14 Inhibits cGAS Degradation Mediated by Selective Autophagy Receptor p62 to Promote Innate Immune Responses. Academic Article uri icon

abstract

  • Cyclic GMP-AMP synthase (cGAS) is an essential DNA virus sensor that triggers type I interferon (IFN) signaling by producing cGAMP to initiate antiviral immunity. However, post-translational regulation of cGAS remains largely unknown. We report that K48-linked ubiquitination of cGAS is a recognition signal for p62-depdendent selective autophagic degradation. The induction of TRIM14 by type I IFN accelerates cGAS stabilization by recruiting USP14 to cleave the ubiquitin chains of cGAS at lysine (K) 414. Knockout of TRIM14 impairs herpes simplex virus type 1 (HSV-1)-triggered antiviral responses in a cGAS-dependent manner. Due to impaired type I IFN production, Trim14-/- mice are highly susceptible to lethal HSV-1 infection. Taken together, our findings reveal a positive feedback loop of cGAS signaling generated by TRIM14-USP14 and provide insights into the crosstalk between autophagy and type I IFN signaling in innate immunity.

published proceedings

  • Mol Cell

altmetric score

  • 6.75

author list (cited authors)

  • Chen, M., Meng, Q., Qin, Y., Liang, P., Tan, P., He, L., ... Cui, J.

citation count

  • 190

complete list of authors

  • Chen, Meixin||Meng, Qingcai||Qin, Yunfei||Liang, Puping||Tan, Peng||He, Lian||Zhou, Yubin||Chen, Yongjun||Huang, Junjiu||Wang, Rong-Fu||Cui, Jun

publication date

  • October 2016