Relationship between (8-14C)adenosine transport and growth inhibition in Neurospora crassa strain ad-8. Academic Article

abstract

• The kinetic parameters of [8-(14)C]adenosine transport by a general nucleoside uptake system were studied in germinated conidia of the ad 8 strain of Neurospora crassa. The apparent K(m) for adenosine uptake by this system was found to be 6.2 muM. The apparent K(i) values for other nucleosides competing with adenosine for uptake were measured by using Dixon plots. Nucleosides which were efficient competitive inhibitors of adenosine transport were found to inhibit severely the rate of growth of strain ad-8 on adenosine-supplemented medium. Xanthosine and thymidine did not inhibit [8-(14)C]adenosine uptake as severely as other nucleosides, nor did they cause significant inhibition of ad-8 growth rate on adenosine.

authors

• Magill, Clint

published proceedings

• J Bacteriol

author list (cited authors)

• Magill, J. M., Spencer, R. R., & Magill, C. W.

citation count

• 11

complete list of authors

• Magill, JM||Spencer, RR||Magill, CW

publication date

• July 1974

publisher

• American Society for Microbiology  Publisher

published in

• Journal of Bacteriology  Journal

keywords

• Adenosine
• Carbon Radioisotopes
• Deoxyribonucleosides
• Deoxyuridine
• Electron Transport
• Guanosine
• Inosine
• Micropore Filters
• Neurospora
• Neurospora Crassa
• Nucleosides
• Spores, Fungal
• Thymidine
• Uridine

PubMed Central ID

• 4276058

Digital Object Identifier (DOI)

• 10.1128/jb.119.1.202-206.1974

start page

• 202

end page

• 206

volume

• 119

issue

• 1

URL

• http://dx.doi.org/10.1128/jb.119.1.202-206.1974