Susceptibility of Mycobacterium tuberculosis Cytochrome bd Oxidase Mutants to Compounds Targeting the Terminal Respiratory Oxidase, Cytochrome c.

abstract

• We deleted subunits I (cydA) and II (cydB) of the Mycobacterium tuberculosis cytochrome bd menaquinol oxidase. The resulting cydA and cydAB mutants were hypersusceptible to compounds targeting the mycobacterial bc1 menaquinol-cytochrome c oxidoreductase and exhibited bioenergetic profiles indistinguishable from strains deficient in the ABC-type transporter, CydDC, predicted to be essential for cytochrome bd assembly. These results confirm CydAB and CydDC as potential targets for drugs aimed at inhibiting a terminal respiratory oxidase implicated in pathogenesis.

authors

• Ioerger, Thomas

published proceedings

• Antimicrob Agents Chemother

altmetric score

• 11.75

author list (cited authors)

• Moosa, A., Lamprecht, D. A., Arora, K., Barry, C. E., Boshoff, H., Ioerger, T. R., ... Warner, D. F.

citation count

• 49

complete list of authors

• Moosa, Atica||Lamprecht, Dirk A||Arora, Kriti||Barry, Clifton E||Boshoff, Helena IM||Ioerger, Thomas R||Steyn, Adrie JC||Mizrahi, Valerie||Warner, Digby F

publication date

• October 2017

publisher

• American Society for Microbiology  Publisher

published in

• Antimicrobial Agents and Chemotherapy  Journal

keywords

• Antitubercular Agents
• Cytochromes C
• Drug Discovery
• Electron Transport Chain
• Electron Transport Complex IV
• Extracellular Flux Analysis
• Genome, Bacterial
• Microbial Sensitivity Tests
• Mycobacterial Respiration
• Mycobacterium Tuberculosis
• Oxidative Phosphorylation
• Oxygen
• Oxygen Consumption
• Sequence Deletion
• Tb Drug Discovery

Digital Object Identifier (DOI)

• 10.1128/AAC.01338-17

start page

• e01338

end page

• e01317

volume

• 61

issue

• 10

URL

• http://dx.doi.org/10.1128/aac.01338-17

user-defined tag

• 3 Good Health and Well-Being