Investigation of the interaction of iron(III) complexes with dAMP by ESI-MS, MALDI-MS and potentiometric titration: insights into synthetic nuclease behavior. Academic Article uri icon


  • We report herein the characterization by electrospray ionization (ESI) mass spectrometry (MS), matrix assisted laser desorption ionization (MALDI-MS) and potentiometric titration of three iron(III) compounds: [Fe(III)(HPClNOL)Cl2]NO3 (1), [Cl(HPClNOL)Fe(III)-(-O)-Fe(III)(HPClNOL)Cl]Cl2H2O (2) and [(SO4)(HPClNOL)Fe(III)-(-O)-Fe(III)(HPClNOL)(SO4)]6H2O (3), where HPClNOL= 1-(bis-pyridin-2-ylmethyl-amino)-3-chloropropan-2-ol). Despite the fact that the compounds have distinct structures in solid state and non-buffered solution, all compounds present similar ESI and MALDI mass spectra in a buffered medium (pH 7.0). At this pH, the species [(PClNOL)Fe(III)-(-O)-Fe(III)(PClNOL)](2+) (m/z 354) was observed for all the compounds under investigation. Potentiometric titration confirms a similar behavior for all compounds, indicating that the dihydroxo form [(OH)(HPClNOL)Fe(III)-(-O)-Fe(III)(HPClNOL)(OH)](2+) is the major species at pH 7.0, for all the compounds. The products of the interaction between compounds (1), (2) and (3) and dAMP (2'-deoxyadenosine-5'-monophosphate) in a buffered medium (pH 7.0) were identified by MALDI-MS/MS. The fragmentation data obtained by MS/MS allow one to identify the nature of the interaction between the iron(III) compounds and dAMP, revealing the direct interaction between the iron center and phosphate groups.

published proceedings

  • Dalton Trans

author list (cited authors)

  • Fernandes, C., Oliveira Moreira, R., Lube, L. M., Horn, A., Szpoganicz, B., Sherrod, S., & Russell, D. H.

citation count

  • 9

complete list of authors

  • Fernandes, Christiane||Oliveira Moreira, Rafaela||Lube, Leonardo M||Horn, Adolfo||Szpoganicz, Bruno||Sherrod, Stacy||Russell, David H

publication date

  • June 2010