Reproducibility and quantitation of matrix-assisted laser desorption ionization mass spectrometry: effects of nitrocellulose on peptide ion yields. Academic Article uri icon

abstract

  • We have observed that using nitrocellulose in sample preparation for matrix-assisted laser desorption ionization (MALDI) time-of-flight mass spectrometry increases the yield of peptide [M+H]+ ions. Addition of nitrocellulose also provides improvement in sample-to-sample reproducibility of MALDI ion yield and improves the precision of peptide quantitation. Mass spectrometry and optical microscopy results suggest that the nitrocellulose modifies the crystallization of matrix/analyte solution to allow more even coverage over the sample surface.

published proceedings

  • Biol Mass Spectrom

altmetric score

  • 3

author list (cited authors)

  • Preston, L. M., Murray, K. K., & Russell, D. H.

citation count

  • 85

complete list of authors

  • Preston, LM||Murray, KK||Russell, DH

publication date

  • September 1993

publisher