AEROSOL MATRIX-ASSISTED LASER-DESORPTION IONIZATION - EFFECTS OF ANALYTE CONCENTRATION AND MATRIX-TO-ANALYTE RATIO Academic Article

abstract

• We have recently developed an aerosol-liquid introduction interface for matrix-assisted laser desorption ionization (MALDI) mass spectrometry. In this study, we examine the effect of matrix-to-analyte ratio and analyte concentration on analyte ion yield. These studies were performed using bradykinin, gramicidin S, bovine insulin, and myoglobin as analytes and -cyano-4-hydroxycinnamic acid and 4-nitroaniline as matrices. The optimum matrix-to-analyte molar ratio for aerosol MALDI was determined to be 10-100:1, which is lower than that typically used for conventional surface MALDI (10010 000:1). The ion yield was found to be a nonlinear function of analyte concentration. Possible explanations for these observations are discussed. 1995, American Chemical Society. All rights reserved.

authors

• Russell, David

published proceedings

• ANALYTICAL CHEMISTRY

author list (cited authors)

• BEESON, M. D., MURRAY, K. K., & RUSSELL, D. H.

citation count

• 23

complete list of authors

• BEESON, MD||MURRAY, KK||RUSSELL, DH

publication date

• July 1995

publisher

• American Chemical Society (ACS)  Publisher

published in

• Analytical Chemistry  Journal

Digital Object Identifier (DOI)

• 10.1021/ac00109a012

start page

• 1981

end page

• 1986

volume

• 67

issue

• 13

URL

• http://dx.doi.org/10.1021/ac00109a012