Sequence and side-chain specific photofragment (193 nm) ions from protonated substance P by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
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abstract
Photodissociation at 193 nm (6.43 eV) of the protonated substance-P, [M + H]+ ions, in a delayed extraction matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometer, is reported. The photofragment ion spectrum of substance P contains a complete series of a-type fragment ions and abundant side-chain cleavage ions. This article focuses on the utility of MALDI-TOF photodissociation for peptide sequencing. Copyright 1999 American Society for Mass Spectrometry.