Sub-femtomole peptide detection in ion mobility-time-of-flight mass spectrometry measurements. Academic Article

abstract

• Sensitivity and detection limit enhancements are obtained for peptides by performing high repetition rate (150 Hz) matrix-assisted laser desorption/ionization (MALDI) coupled with ion mobility-time-of-flight mass spectrometry. Absolute limits of detection (3sigma) for model peptides are on the order of 0.1 fmol of peptide deposited and represent a factor of 40-60 improvement over data obtained using typical low repetition (20 Hz) MALDI. This increase in sensitivity is demonstrated for two-dimensional MALDI-IM-TOFMS peptide mass mapping of bovine hemoglobin.

authors

• Russell, David

published proceedings

• J Proteome Res

author list (cited authors)

• McLean, J. A., & Russell, D. H.

citation count

• 33

complete list of authors

• McLean, John A||Russell, David H

publication date

• May 2003

publisher

• American Chemical Society (ACS)  Publisher

published in

• Journal of Proteome Research  Journal

keywords

• Angiotensin I
• Angiotensin II
• Animals
• Cattle
• Hemoglobins
• Humans
• Peptide Fragments
• Sensitivity And Specificity
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

PubMed Central ID

• 12938932

Digital Object Identifier (DOI)

• 10.1021/pr034004p

start page

• 427

end page

• 430

volume

• 2

issue

• 4