Severely Compromised Anabolic Response to Nutrition in a Pseudomonas aeroginosa (PM) Induced Hyper-dynamic Sepsis-Recovery Pig Model Conference Paper uri icon


  • RationaleFeeding patients with sepsis is critical to restore muscle mass. However, it is unknown whether nutrition is able to induce an anabolic response during the early treatment phase of sepsis. We developed a reversible sepsis model in catheterized pigs by infusing live bacteria (PM) IV, followed by antibiotic induced recovery. We assessed the anabolic response to amino acid (AA) nutrition in the early recovery phase of sepsis using stable isotope methodology by measuring whole body protein synthesis (WbPS), protein breakdown (WbPB), net protein synthesis (netPS) and the efficiency of conversion of AA intake to protein (ProtConv).MethodIn 25 pigs (25 kg), IV PM (3e8 CFU/ml/h) induced acute severe sepsis (n=13) or control IV solution (n=12) for 6 hours was followed by a single dose of gentamycine (5 mg/kg bw) and intragastric constant continuous feeding with a dextrose containing AA mixture (pig muscle AA profile, 31 mg N/kg bw/h, 30% daily intake) for 6 hours. We measured WbPB, WbPS, net PS and ProtConv using primed, constant continuous intravenous infusion of Phenylalanine(ring2H5) and Tyrosine(13C9, 15N) stable isotopes and collected arterial blood samples between 912h. Enrichments of AA plasma pools were analyzed by LCMS/MS. Data are expressed as mean (SE) and stats by twoway ANOVA or Student ttest using Graphpad Prism6.ResultsIn the sepsis group during the AA nutrition intervention, WbPB rate was higher (p<0.0001), netPS rate was lower (p<0.0001), but no changes in WbPS (p=0.989). The ProtConv fraction was lower in the sepsis than in the control group (p<0.0001). On average ProtConv was reduced with 43%: 65.1(3.6) vs 36.2(4.3)%, (p<0.0001).ConclusionThe whole body anabolic response to AA nutrition is severely compromised in the early treatment phase of acute severe sepsis. Our innovative pig sepsisrecovery model enables examination of the anabolic capacity of modified diets to restore sepsis induced muscle loss.Support or Funding InformationThis study was supported by NIH R01GM084447 and S10RR027047

published proceedings


author list (cited authors)

  • Ten Have, G. A., Engelen, M. P., Wolfe, R. R., & Deutz, N. E.

citation count

  • 0

complete list of authors

  • Ten Have, Gabriella A||Engelen, Marielle P||Wolfe, Robert R||Deutz, Nicolaas E

publication date

  • April 2016