Muscle breakdown determines Arginine (ARG) availability during hyperdynamic sepsis in the pig Conference Paper uri icon

abstract

  • RATIONALEArginine availability plays a key role in systemic ARG to nitric oxide (NO) conversion, but in sepsis it is unknown which of the organs contribute.METHODSTransorgan ARG kinetics was studied in 12 pigs with Pseudomonas aeroginosa induced hyperdynamic sepsis and 8 controls, using primedcontinuous Phenylalanine (13C6), ARG (guanido15N2) and Citrulline (CIT) (ureido13C5,52H2) infusion. 18 h after start of the sepsis induction, whole body protein breakdown (WbPB), WbArg, WbCit, ARG de novo (WbdenovoArg) and nitric oxide (WbNO) production, and transorgan net balances (NB), disposal and production were measured. Enrichment and concentrations were analysed by LCMS. Data were reflected as mean (SE) (in nmol/kg bw/min); stats was done by RMANOVA.RESULTSSepsis increased WbPB, WbArg, WbNO (p<0.05), but did not change WbdenovoArg and WbCit. Only muscle ARG NB changed from net uptake to net release in the PM pigs (115(26) to 127(58); p=0.0046) and was caused by increased ARG production (326(157) to 762(107), p=0.028) and associated with increased muscle protein breakdown. No changes in gut, liver and lung ARG metabolism were observed.CONCLUSIONARG availability during sepsis is maintained only by stimulated muscle protein breakdown.

published proceedings

  • FASEB JOURNAL

author list (cited authors)

  • Ten Have, G. A., Engelen, M. P., Wolfe, R. R., & Deutz, N. E.

citation count

  • 1

complete list of authors

  • Ten Have, Gabriella A||Engelen, Marielle P||Wolfe, Robert R||Deutz, Nicolaas E

publication date

  • April 2012

publisher