Complete nucleotide sequences and virion particle association of two satellite RNAs of panicum mosaic virus. Academic Article uri icon


  • Over six decades ago, panicum mosaic virus (PMV) was identified as the first viral pathogen of cultivated switchgrass (Panicum virgatum). Subsequently, PMV was demonstrated to support the replication of both a satellite RNA virus (SPMV) and satellite RNA (satRNA) agents during natural infections of host grasses. In this study, we report the isolation and full-length sequences of two PMV satRNAs identified in 1988 from St. Augustinegrass (Stenotaphrum secundatum) and centipedegrass (Eremochloa ophiuroides) hosts. Each of these satellites have sequence relatedness at their 5'- and 3'-ends. In addition, satC has a region of 100 nt complementary to the 3'-end of the PMV genome. These agents are associated with purified virions of SPMV infections. Additionally, satS and satC RNAs contain conserved in-frame open reading frames in the complementary-sense sequences that could potentially generate 6.6- and 7.9-kDa proteins, respectively. In protoplasts and plants satS is infectious, when co-inoculated with the PMV RNA alone or PMV+SPMV RNAs, and negatively affects their accumulation.

published proceedings

  • Virus Res

altmetric score

  • 0.25

author list (cited authors)

  • Pyle, J. D., Monis, J., & Scholthof, K.

citation count

  • 10

complete list of authors

  • Pyle, Jesse D||Monis, Judit||Scholthof, Karen-Beth

publication date

  • January 2017