The flippase DnfB is cargo of fimbrin-associated endocytosis in Aspergillus nidulans, and likely recycles through the late Golgi
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© 2016 Zachary Schultzhaus and Brian D. Shaw. Filamentous fungi exhibit rapid, polarized growth governed by precise segregation of endocytosis and exocytosis at the site of cell expansion. Endocytosis, in particular, is concentrated at a zone termed the endocytic collar, surrounding the cell tip, where secretory vesicle fusion occurs. How the separation of these processes is established and maintained is still unclear, but one possible purpose for this organization is the ability of endocytosis to remove and recycle proteins that diffuse away from the site of growth. The phospholipid flippases DnfA and DnfB localize to sites of growth and endocytosis in the fungus Aspergillus nidulans, and may be involved in secretion. DnfA is endocytosed in part through an NPFxD motif, but whether DnfB is endocytosed or recycled is unknown. Here, we demonstrate that DnfB is also endocytosed, and that recycling through the late Golgi maintains its organization in the growing tip.
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Schultzhaus, Z., & Shaw, B. D.