Biosynthesis of the thiamin-thiazole in eukaryotes: identification of a thiazole tautomer intermediate. Academic Article

abstract

• Thiamin thiazole biosynthesis in eukaryotes is still not completely understood. In this report, a late intermediate, tightly bound to the active site of the Saccharomyces cerevisiae thiazole synthase, was identified as an adenylated thiazole tautomer. The reactivity of this unusual compound was evaluated. Its identification provides an additional molecular snapshot of the complex reaction sequence catalyzed by the eukaryotic thiazole synthase and identifies the final step of the thiamin-thiazole biosynthesis.

authors

• Begley, Tadhg

published proceedings

• J Am Chem Soc

author list (cited authors)

• Chatterjee, A., Schroeder, F. C., Jurgenson, C. T., Ealick, S. E., & Begley, T. P.

citation count

• 24

complete list of authors

• Chatterjee, Abhishek||Schroeder, Frank C||Jurgenson, Christopher T||Ealick, Steven E||Begley, Tadhg P

publication date

• August 2008

publisher

• American Chemical Society (ACS)  Publisher

published in

• Journal of the American Chemical Society  Journal

keywords

• Adenosine Diphosphate
• Catalysis
• Catalytic Domain
• Chromatography, High Pressure Liquid
• Eukaryotic Cells
• Isomerism
• Models, Biological
• Saccharomyces Cerevisiae
• Spectrum Analysis
• Thiamine
• Thiazoles
• Time Factors

PubMed Central ID

• 18652458

Digital Object Identifier (DOI)

• 10.1021/ja802140a

start page

• 11394

end page

• 11398

volume

• 130

issue

• 34

URL

• http://dx.doi.org/10.1021/ja802140a