Production of an interspecific hybrid between Brassica fruticulosa and B. rapa
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An interspecific hybrid between a wild species, Brassica fruticulosa (2n = 16, FF) and a crop Brassica species, B. rapa (2n = 20, AA) was synthesized using sequential ovary-ovule culture on MS medium supplemented with casein hydrolysate. Morphological, molecular and cytogenetic analysis confirmed the true hybrid nature of the offspring. The F 1 plants (2n = 18) were intermediate in morphology, highly pollen-sterile as well as self-sterile. A maximum of three bivalents per PMC was recorded, but 14 I + 2 II was the most common meiotic configuration. Normal pollen fertility and regular bivalent (18 II) formation was observed in the amphiploid sectors of the hybrid plants. The F 1 hybrid harboured significantly lower aphid populations than the crop Brassica parent.