Fluorometric evidence for the binding of cholesterol to the filipin complex. Academic Article

abstract

• The data show that the filipin complex in water has a characteristic fluorescence spectrum with a broad emission maxima at 497 nm. Addition of cholesterol to the aqueous solution decreases the absorbance, the corrected fluorescence, and the partial quantum efficiency. The reduction in partial quantum efficiency, which is independent of the concentration of filipin, is definitive evidence that the filipin complex interacts with sterols in aqueous solution. The data indicate that changes in fluorescence may be a sensitive tool for monitoring the interaction of filipins with sterols. 1971, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.

authors

• Schroeder, Friedhelm

published proceedings

• J Antibiot (Tokyo)

altmetric score

• 3

author list (cited authors)

• Schroeder, F., Holland, J. F., & Bieber, L. L.

citation count

• 36

complete list of authors

• Schroeder, F||Holland, JF||Bieber, LL

publication date

• December 1971

publisher

• Japan Antibiotics Research Association  Publisher

published in

• Journal of Antibiotics: an international journal devoted to research on bioactive microbial products  Journal

keywords

• Anti-Bacterial Agents
• Cholesterol
• FLUORESCENCE
• Spectrophotometry

PubMed Central ID

• 5140531

Digital Object Identifier (DOI)

• 10.7164/antibiotics.24.846

start page

• 846

end page

• 849

volume

• 24

issue

• 12

URL

• http://dx.doi.org/10.7164/antibiotics.24.846