The detection of heat-aggregated IgG (as a model for immune complexes) by reverse passive haemagglutination using a human monoclonal rheumatoid factor coupled to erythrocytes. Academic Article uri icon

abstract

  • A human monoclonal IgM rheumatoid factor (RF) produced in vitro by an Epstein-Barr virus (EBV)-immortalized cell line was purified by protein A-Sepharose adsorption and coupled by the chromic chloride method to human erythrocytes. The RF-coupled cells were incorporated in reverse passive haemagglutination (RPH) assays to detect immune complexes (IC) using heat-aggregated human IgG as a model system. The sensitivity of the RPH was comparable to an enzyme-linked immunosorbent assay (ELISA) using sheep C1q for the detection of ICs.

published proceedings

  • J Immunol Methods

author list (cited authors)

  • Welsh, C. J., Steinitz, M., Clark, M. R., & Coombs, R. R.

citation count

  • 0

complete list of authors

  • Welsh, CJ||Steinitz, M||Clark, MR||Coombs, RR

publication date

  • January 1987