Single-shot detection of bacterial endospores via coherent Raman spectroscopy.
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Recent advances in coherent Raman spectroscopy hold exciting promise for many potential applications. For example, a technique, mitigating the nonresonant four-wave-mixing noise while maximizing the Raman-resonant signal, has been developed and applied to the problem of real-time detection of bacterial endospores. After a brief review of the technique essentials, we show how extensions of our earlier experimental work [Pestov D, et al. (2007) Science 316:265-268] yield single-shot identification of a small sample of Bacillus subtilis endospores (approximately 10(4) spores). The results convey the utility of the technique and its potential for "on-the-fly" detection of biohazards, such as Bacillus anthracis. The application of optimized coherent anti-Stokes Raman scattering scheme to problems requiring chemical specificity and short signal acquisition times is demonstrated.
author list (cited authors)
Pestov, D., Wang, X. i., Ariunbold, G. O., Murawski, R. K., Sautenkov, V. A., Dogariu, A., Sokolov, A. V., & Scully, M. O.
complete list of authors
Pestov, Dmitry||Wang, Xi||Ariunbold, Gombojav O||Murawski, Robert K||Sautenkov, Vladimir A||Dogariu, Arthur||Sokolov, Alexei V||Scully, Marlan O