Single-shot detection of bacterial endospores via coherent Raman spectroscopy. Academic Article uri icon


  • Recent advances in coherent Raman spectroscopy hold exciting promise for many potential applications. For example, a technique, mitigating the nonresonant four-wave-mixing noise while maximizing the Raman-resonant signal, has been developed and applied to the problem of real-time detection of bacterial endospores. After a brief review of the technique essentials, we show how extensions of our earlier experimental work [Pestov D, et al. (2007) Science 316:265-268] yield single-shot identification of a small sample of Bacillus subtilis endospores (approximately 10(4) spores). The results convey the utility of the technique and its potential for "on-the-fly" detection of biohazards, such as Bacillus anthracis. The application of optimized coherent anti-Stokes Raman scattering scheme to problems requiring chemical specificity and short signal acquisition times is demonstrated.

published proceedings

  • Proc Natl Acad Sci U S A

altmetric score

  • 6

author list (cited authors)

  • Pestov, D., Wang, X. i., Ariunbold, G. O., Murawski, R. K., Sautenkov, V. A., Dogariu, A., Sokolov, A. V., & Scully, M. O.

citation count

  • 110

complete list of authors

  • Pestov, Dmitry||Wang, Xi||Ariunbold, Gombojav O||Murawski, Robert K||Sautenkov, Vladimir A||Dogariu, Arthur||Sokolov, Alexei V||Scully, Marlan O

publication date

  • January 2008