Progress in Gene Editing Transgenesis Genome Manipulation in Mosquitoes Chapter

abstract

• 2016 Elsevier Ltd Recent advances in site-specific gene editing technologies, split genetic systems, recombinases and transposon remobilization have been applied to disease vector mosquitoes with great success. These in turn now enable a suite of hypothesis-driven questions concerning the physiology and behaviour of important mosquito species, guided by previous advances in genome sequencing and transcriptomics. In this chapter, we will review progress in CRISPR/Cas9-based gene disruption, including target selection, mutation identification, tracking homology-dependent gene insertion, CRISPR-mediated gene drive mechanisms and CRISPR/Cas9 variants. We will also review progress in using split genetic systems such as Gal4/UAS, recombinase-mediated cassette exchange using C31, as well as piggyBac-mediated forward genetic screens using transgene remobilization.

authors

• Adelman, Zachary

author list (cited authors)

• Overcash, J., & Adelman, Z. N.

citation count

• 0

complete list of authors

• Overcash, J||Adelman, ZN

Book Title

• PROGRESS IN MOSQUITO RESEARCH

publication date

• January 2016

publisher

• Elsevier  Publisher

published in

• Advances in Insect Physiology  Journal

Digital Object Identifier (DOI)

• 10.1016/bs.aiip.2016.05.001

International Standard Book Number (ISBN) 13

• 9780128024577

start page

• 1

end page

• 35

volume

• 51

URL

• http://dx.doi.org/10.1016/bs.aiip.2016.05.001