Methods for TALEN Evaluation, Use, and Mutation Detection in the Mosquito Aedes aegypti. Chapter

abstract

• The generation and study of transgenic Aedes aegypti mosquitoes provides an essential tool for elucidating the complex molecular biology of this important vector. Within the field, genetic manipulation has surpassed the proof of principle stage and is now utilized in both applied and theoretical vector control strategies. The application of new instruments, technologies and techniques allows ever more controlled experiments to be conducted. In this text we describe microinjection of Ae. aegypti embryos in the context of evaluating and performing genomic editing with transcription activator-like effector nucleases (TALENs).

authors

• Adelman, Zachary
• Myles, Kevin

author list (cited authors)

• Basu, S., Aryan, A., Haac, M. E., Myles, K. M., & Adelman, Z. N.

citation count

• 8

complete list of authors

• Basu, Sanjay||Aryan, Azadeh||Haac, Mary Etna||Myles, Kevin M||Adelman, Zach N

Book Title

• TALENS: METHODS AND PROTOCOLS

publication date

• January 2016

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Aedes
• Animals
• Animals, Genetically Modified
• DNA Mutational Analysis
• Dengue
• Dengue Virus
• Endonucleases
• Gene Editing
• Genome
• HRMA
• Insect Vectors
• Mosquito
• Mutagenesis
• Mutation
• Nuclease
• Rna Editing
• Talen

PubMed Central ID

• 26443221

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-2932-0_13

International Standard Book Number (ISBN) 13

• 978-1-138-82580-2

start page

• 157

end page

• 177

volume

• 1338

URL

• http://dx.doi.org/10.1007/978-1-4939-2932-0_13

user-defined tag

• 3 Good Health and Well-Being