Effects of purified components of jellyfish toxin (Stomolophus meleagris) on active sodium transport Academic Article uri icon

abstract

  • Using preparative isoelectric focusing and discontinuous membrane partition chromatography, three fractions which alter sodium transport were isolated from venom of the jellyfish Stomolophus meleagris. A fraction which inhibited sodium transport was partially purified and found to possess a molecular weight of approximately 50,000 and an isoelectric point of 3·30. This component, which appeared to affect active sodium transport at the enzyme level, was also found to produce hemorrhage and necrosis in mice. Two fractions were purified which increased sodium transport in a passive manner and possessed molecular weights of 10,000-50,000, and greater than 300,000, respectively, and have isoelectric points of 4·15 and 5·30. The fraction of 300,000 mol. wt contained most of the toxicity. It is concluded that the immediate pharmacological response to jellyfish toxin possibly is due to components which increase sodium transport, while the apparent delayed response is probably due to a component of the toxin which acts in a cardiotonic, ouabain-like manner. © 1975.

author list (cited authors)

  • Toom, P. M., & Phillips, T. D.

citation count

  • 12

complete list of authors

  • Toom, PM||Phillips, TD

publication date

  • August 1975