Dual-Probe Luminescence Lifetime Measurements for the Oxygen Compensation in Enzymatic Biosensors
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abstract
An optical system for monitoring glucose with a means of compensating for local oxygen variations is described. Measurements of sensor response to glucose under varying oxygen levels indicate a strong dependence of the optical response on ambient oxygen. The two-probe approach enables simultaneous monitoring of luminescence lifetime of microparticle sensors with a compact optical system. Glucose tracking experiments with both probes reveal a modulation of oxygen in the vicinity of sensors, even when external oxygen is constant. These results prove the concept of simultaneous monitoring of multiple sensor types in the same matrix. 2009 IEEE.