Wide-field extrinsic optical signal imaging of fluorescence potassium sensors
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abstract
Wide-field extrinsic optical signal (EOS) imaging is an excellent method for its spatial resolution of 50-100m and a wide imaging field of several mm 2 that cannot be achieved by any microscope. Fluorescence images of a nanoengineered sensor for potassium, fabricated by using electrostatic layer-by-layer (LbL) self-assembly technique, were collected by a wide-field EOS imaging system under different concentrations of [K +]. The images were analyzed using MATLAB to study the quenching properties of the potassium nanosensor. 2006 IEEE.