Stable encapsulation of active enzyme by application of multilayer nanofilm coatings to alginate microspheres. Academic Article

abstract

• In an effort to improve the stability for long-term biosensor use, layer-by-layer self-assembly was explored as a potential technique to provide a diffusion barrier to encapsulated glucose oxidase inside alginate microspheres (<5 microm), fabricated using an emulsification technique. The total loss of encapsulated enzyme was reduced to less than 25 and 15% with the application of single PAH/PSS and crosslinked PAH/PAA coatings, respectively, in comparison to at least a 45% loss observed with uncoated and PDDA/PSS-coated microspheres. Furthermore, it was found that enzyme within PAH/PSS- and crosslinked PAH/PAA-coated spheres retained more than 84 and 60% of initial activity, respectively, after three months, whereas uncoated and PDDA/PSS-coated microspheres retained less than 20%.

authors

• McShane, Michael

published proceedings

• Macromol Biosci

altmetric score

• 3

author list (cited authors)

• Srivastava, R., Brown, J. Q., Zhu, H., & McShane, M. J.

citation count

• 76

complete list of authors

• Srivastava, Rohit||Brown, Jonathan Q||Zhu, Huiguang||McShane, Michael J

publication date

• August 2005

publisher

• Wiley  Publisher

published in

• Macromolecular Bioscience  Journal

keywords

• Alginates
• Aspergillus Niger
• Biosensing Techniques
• Enzymes, Immobilized
• Fungal Proteins
• Glucose Oxidase
• Glucuronic Acid
• Hexuronic Acids
• Microspheres
• Nanotechnology

PubMed Central ID

• 16096991

Digital Object Identifier (DOI)

• 10.1002/mabi.200500061

start page

• 717

end page

• 727

volume

• 5

issue

• 8

URL

• http://dx.doi.org/10.1002/mabi.200500061