Dissolved core alginate microspheres as "smart-tattoo" glucose sensors. Academic Article

abstract

• The feasibility of multilayer thin film coated dissolved-core alginate-templated microsphere sensors based on fluorescence resonance energy transfer and competitive binding, was explored in simulated interstitial fluid, using glucose as a model analyte. The glucose sensitivity was observed to be 0.89%/mM glucose with a linear response in the range of 0-50 mM glucose. The sensor response was observed to be completely reversible in nature with a response time of 120 seconds. The system was further demonstrated to respond similarly using near-infrared dyes (Alexa Fluor-647-labeled dextran as donor and QSY-21-conjugated apo-GOx as acceptor) which exhibited a sensitivity of 0.94%/mM glucose with a linear response in range of 0-50 mM glucose, making the sensor more amenable to in vivo use, when implanted in scattering tissue.

authors

• McShane, Michael

published proceedings

• Annu Int Conf IEEE Eng Med Biol Soc

author list (cited authors)

• Chaudhary, A., Raina, M., McShane, M. J., & Srivastava, R.

citation count

• 1

complete list of authors

• Chaudhary, Ayesha||Raina, Monica||McShane, Michael J||Srivastava, Rohit

publication date

• September 2009

publisher

• IEEE  Publisher

published in

• n2375-7477ISSN  Journal

keywords

• Alginates
• Biomedical Engineering
• Biosensing Techniques
• Cyclic AMP
• Equipment Design
• Extracellular Fluid
• Fluorescence Resonance Energy Transfer
• Glucose
• Glucose Oxidase
• Glucuronic Acid
• Hexuronic Acids
• Humans
• Microscopy, Electron, Scanning
• Microspheres
• Spectrophotometry
• Spectroscopy, Near-Infrared
• Time Factors

Digital Object Identifier (DOI)

• 10.1109/IEMBS.2009.5334597

start page

• 4098

end page

• 4101

volume

• 2009

URL

• http://dx.doi.org/10.1109/iembs.2009.5334597