Left-Handed Helical Ribbon Intermediates in the Self-Assembly of a β-Sheet Peptide Academic Article uri icon


  • We report the observation of intermediate structures in the self-assembly of the peptide KFE8 (FKFEFKFE), designed with alternating polar and nonpolar amino acids. Self-assembly was followed over time using atomic force microscopy (AFM), transmission electron microscopy (TEM), and circular dichroism (CD). Molecular dynamics simulations suggest that these intermediates are left-handed double helical β-sheets. These findings have implications in the study of β-sheet fibril formation, and in the molecular design of materials.

author list (cited authors)

  • Marini, D. M., Hwang, W., Lauffenburger, D. A., Zhang, S., & Kamm, R. D.

publication date

  • January 1, 2002 11:11 AM