ConA-based glucose sensing using the long-lifetime Azadioxatriangulenium fluorophore Conference Paper uri icon


  • Fluorescent glucose sensing technologies have been identified as possible alternatives to current continuous glucose monitoring approaches. We have recently introduced a new, smart fluorescent ligand to overcome the traditional problems of ConA-based glucose sensors. For this assay to be translated into a continuous glucose monitoring device where both components are free in solution, the molecular weight of the smart fluorescent ligand must be increased. We have identified ovalbumin as a naturally-occurring glycoprotein that could serve as the core-component of a 2nd generation smart fluorescent ligand. It has a single asparagine residue that is capable of displaying an N-linked glycan and a similar isoelectric point to ConA. Thus, binding between ConA and ovalbumin can potentially be monovalent and sugar specific. This work is the preliminary implementation of fluorescently-labeled ovalbumin in the ConA-based assay. We conjugate the red-emitting, long-lifetime azadioxatriangulenium (ADOTA+) dye to ovalbumin, as ADOTA have many advantageous properties to track the equilibrium binding of the assay. The ADOTA-labeled ovalbumin is paired with Alexa Fluor 647-labeled ConA to create a Frster Resonance Energy Transfer (FRET) assay that is glucose dependent. The assay responds across the physiologically relevant glucose range (0-500 mg/dL) with increasing intensity from the ADOTA-ovalbumin, showing that the strategy may allow for the translation of the smart fluorescent ligand concept into a continuous glucose monitoring device. 2014 SPIE.

name of conference

  • Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics

published proceedings


author list (cited authors)

  • Cummins, B., Simpson, J., Gryczynski, Z., Sorensen, T. J., Laursen, B. W., Graham, D., Birch, D., & Cote, G.

citation count

  • 2

complete list of authors

  • Cummins, Brian||Simpson, Jonathan||Gryczynski, Zygmunt||Sorensen, Thomas Just||Laursen, Bo W||Graham, Duncan||Birch, David||Cote, Gerard

editor list (cited editors)

  • Coté, G. L.

publication date

  • January 2014