An ALV-J isolate is responsible for spontaneous haemangiomas in layer chickens in China. Academic Article uri icon

abstract

  • Subgroup J avian leukosis virus (ALV-J), first isolated in 1989, mainly induces tumours of myeloid leukosis (ML) in meat-type chickens. In 2006, ALV-J strain SCAU-HN06 was isolated from commercial layer hens with spontaneous haemangiomas in China. To confirm its role in the induction of haemangioma, we constructed a full-length copy of the proviral genome from SCAU-HN06, recovered virus from DF-1 cells detected by enzyme-linked immunosorbent assay, characterized its growth property and investigated its pathogenicity. The recovered virus appeared to be identical to SCAU-HN06 analysed by both blast gene sequences and indirect immunofluorescence assay. It also showed similarities in growth to the parental wild-type virus in vitro. The pathogenicity of the rescued and parental virus in specific-pathogen-free White Leghorn chickens was investigated. Both SCAU-HN06 and rSCAU-HN06 could induce haemangioma, with incidence of 52% and 42.8% respectively. Overall, our findings indicated that the ALV-J strain SCAU-HN06 was the causal agent inducing haemangiomas rather than ML in certain layer chickens.

published proceedings

  • Avian Pathol

author list (cited authors)

  • Zhang, H., Lai, H., Qi, Y., Zhang, X., Ning, Z., Luo, K., ... Liao, M.

citation count

  • 16

complete list of authors

  • Zhang, He-nan||Lai, Han-zhang||Qi, Yan||Zhang, Xiao-tao||Ning, Zhang-yong||Luo, Kai-jian||Xin, Chao-an||Cao, Wei-sheng||Liao, Ming

publication date

  • June 2011