Baculovirus-insect cell production of bioactive choriogonadotropin-immunoglobulin G heavy-chain fusion proteins in sheep. Academic Article

abstract

• A hybrid cDNA encoding a fusion protein between the beta subunit of hCG (beta hCG) and constant domains of a mouse IgG heavy chain (CH1-3) was inserted into a baculovirus expression vector. Insect cells transfected with foreign DNA synthesized multimeric forms of fusion protein that inhibited hCG-induced steroid hormone secretion by mouse Leydig tumor cells. Leydig cells were lysed by beta hCG-CH1-3 in the presence of complement. Intravenous injection of beta hCG-CH1-3 in rams was associated with testicular mononuclear leukocyte infiltration, interstitial tissue damage, and a transient depression in circulatory testosterone (levels returned to normal within 2 wk). It appears that targeted cell-killing can be mediated by recombinant proteins composed of the receptor-binding moiety of hormones and truncated effector (Fc) regions of lethal antibodies.

authors

• Johnson, Gregory

published proceedings

• Biol Reprod

altmetric score

• 9

author list (cited authors)

• Johnson, G. A., Hansen, T. R., Austin, K. J., Van Kirk, E. A., & Murdoch, W. J.

citation count

• 1

complete list of authors

• Johnson, GA||Hansen, TR||Austin, KJ||Van Kirk, EA||Murdoch, WJ

publication date

• January 1995

publisher

• Oxford University Press (OUP)  Publisher

published in

• Biology of Reproduction  Journal

keywords

• Animals
• Base Sequence
• Cell Line
• Chorionic Gonadotropin
• DNA Primers
• DNA, Complementary
• Immunoglobulin G
• Immunoglobulin Heavy Chains
• Male
• Mice
• Molecular Sequence Data
• Nucleopolyhedroviruses
• Recombinant Fusion Proteins
• Sheep
• Spodoptera
• Testis
• Testosterone
• Tumor Cells, Cultured

PubMed Central ID

• 7711185

Digital Object Identifier (DOI)

• 10.1095/biolreprod52.1.68

start page

• 68

end page

• 73

volume

• 52

issue

• 1