Baculovirus-insect cell production of bioactive choriogonadotropin-immunoglobulin G heavy-chain fusion proteins in sheep. Academic Article uri icon


  • A hybrid cDNA encoding a fusion protein between the beta subunit of hCG (beta hCG) and constant domains of a mouse IgG heavy chain (CH1-3) was inserted into a baculovirus expression vector. Insect cells transfected with foreign DNA synthesized multimeric forms of fusion protein that inhibited hCG-induced steroid hormone secretion by mouse Leydig tumor cells. Leydig cells were lysed by beta hCG-CH1-3 in the presence of complement. Intravenous injection of beta hCG-CH1-3 in rams was associated with testicular mononuclear leukocyte infiltration, interstitial tissue damage, and a transient depression in circulatory testosterone (levels returned to normal within 2 wk). It appears that targeted cell-killing can be mediated by recombinant proteins composed of the receptor-binding moiety of hormones and truncated effector (Fc) regions of lethal antibodies.

published proceedings

  • Biol Reprod

altmetric score

  • 9

author list (cited authors)

  • Johnson, G. A., Hansen, T. R., Austin, K. J., Van Kirk, E. A., & Murdoch, W. J.

citation count

  • 1

complete list of authors

  • Johnson, GA||Hansen, TR||Austin, KJ||Van Kirk, EA||Murdoch, WJ

publication date

  • January 1995