Toward regulation of gonadal function by a synthetic hybrid molecule composed of gonadotropin and Fc fragment of immunoglobulin G. Academic Article

abstract

• A conjugate of human chorionic gonadotropin (HCG) and Fc fragment of immunoglobulin G was prepared by covalent cross-linking using the heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio) propionate. Mouse Leydig tumor cells expressing receptors for luteinizing hormone were specifically lysed in vitro as a consequence of complement fixation via the Fc component of the hybrid molecule. Furthermore, administration of HCG-Fc to rams caused an acute depression in circulatory testosterone. This novel concept of targeted inhibition of gonadal function could prove to have future applications in control of reproductive processes.

authors

• Johnson, Gregory

published proceedings

• Am J Reprod Immunol

altmetric score

• 3

author list (cited authors)

• Johnson, G. A., Wilken, C., Van Kirk, E. A., Belden, E. L., & Murdoch, W. J.

citation count

• 13

complete list of authors

• Johnson, GA||Wilken, C||Van Kirk, EA||Belden, EL||Murdoch, WJ

publication date

• May 1990

publisher

• Wiley  Publisher

published in

• American Journal of Reproductive Immunology  Journal

keywords

• Animals
• Cell Survival
• Chorionic Gonadotropin
• Cross-Linking Reagents
• Immunoglobulin Fc Fragments
• Immunoglobulin G
• Leydig Cells
• Luteinizing Hormone
• Male
• Reproduction
• Sheep
• Testis
• Testosterone
• Tumor Cells, Cultured

PubMed Central ID

• 2397039

Digital Object Identifier (DOI)

• 10.1111/j.1600-0897.1990.tb00663.x

start page

• 22

end page

• 25

volume

• 23

issue

• 1

URL

• http://dx.doi.org/10.1111/j.1600-0897.1990.tb00663.x