Bovine granulocyte chemotactic protein-2 is secreted by the endometrium in response to interferon-tau (IFN-tau). Academic Article uri icon


  • Interferon-tau (IFN-tau) is secreted by the bovine conceptus and may regulate synthesis of uterine endometrial cytokines to provide an environment that is conductive to embryo development and implantation. Interferon-tau stimulates secretion of an 8-kDa uterine protein (P8) in the cow. P8 was purified, digested to yield internal peptides, and partially sequenced to determine identity. Two internal peptides had 100% (13-mer) and 92% (12-mer) amino acid sequence identity with bovine granulocyte chemotactic protein-2 (bGCP-2). Bovine GCP-2 is an alpha-chemokine that acts primarily as a potent chemoattractant for granulocyte cells of the immune system. A peptide was synthesized based on a region of bGCP-2 that overlapped with a P8 peptide amino acid sequence, coupled to keyhole limpet hemocyanin, and used to generate high titer polyclonal antiserum in sheep. Western blots revealed that bGCP-2 was not released by endometrium from day 14 nonpregnant cows, but was released in response to 25 nM IFN-tau (p<0.05). Uterine GCP-2 exhibited high affinity to heparin agarose, a characteristic shared by all alpha chemokines. This is the first report describing presence of GCP-2 in the uterine endometrium and regulation by IFN-tau. The regulation of bGCP-2 by IFN-tau may have important implications for cytokine networking in the uterus during pregnancy. Also, the regulation of inflammation and angiogenesis by bGCP-2 working together with other cytokines may be integral to establishing early pregnancy and implantation in the cow.

published proceedings

  • Endocrine

altmetric score

  • 3

author list (cited authors)

  • Teixeira, M. G., Austin, K. J., Perry, D. J., Dooley, V. D., Johnson, G. A., Francis, B. R., & Hansen, T. R.

citation count

  • 41

complete list of authors

  • Teixeira, MG||Austin, KJ||Perry, DJ||Dooley, VD||Johnson, GA||Francis, BR||Hansen, TR

publication date

  • January 1997