Generation of trophoblast stem cells. Academic Article

abstract

• The isolation and culture of both embryonic and extraembryonic stem cells provide an enormous opportunity to study the molecular processes that establish and maintain lineage-specific, monoallelic patterns of gene expression. This chapter describes the isolation an culture of trophectoderm stem cells from mouse blastocyst stage embryos. Using this powerful in vitro system, scientists can now begin to tease apart the epigenetic processes that result in placental patterns of imprinted gene expression and begin to better understand the role these genes play in development and disease.

authors

• Golding, Michael

published proceedings

• Methods Mol Biol

author list (cited authors)

• Golding, M. C.

citation count

• 2

complete list of authors

• Golding, Michael C

publication date

• October 2012

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Cell Culture Techniques
• Cell Line
• Cell Separation
• Cryopreservation
• Culture Media, Conditioned
• Female
• Fibroblasts
• Mice
• Mitomycin
• Stem Cells
• Trophoblasts

PubMed Central ID

• 22907489

Digital Object Identifier (DOI)

• 10.1007/978-1-62703-011-3_3

start page

• 49

end page

• 59

volume

• 925