Experimental and computational laser tissue welding using a protein patch. Academic Article uri icon


  • An in vitro study of laser tissue welding mediated with a dye-enhanced protein patch was conducted. Fresh sections of porcine aorta were used for the experiments. Arteriotomies were treated using an indocyanine green dye-enhanced collagen patch activated by an 805-nm continuous-wave fiber-delivered diode laser. Temperature histories of the surface of the weld site were obtained using a hollow glass optical fiber-based two-color infrared thermometer. The experimental effort was complemented by simulations with the LATIS (LAser-TISsue) computer code, which uses coupled Monte Carlo, thermal transport, and mass transport models. Comparison of simulated and experimental thermal data indicated that evaporative cooling clamped the surface temperature of the weld site below 100C. For fluences of approximately 200J/cm2, peak surface temperatures averaged 74C and acute burst strengths consistently exceeded 0.14106dyn/cm (hoop tension). The combination of experimental and simulation results showed that the inclusion of water transport and evaporative losses in the computer code has a significant impact on the thermal distributions and hydration levels throughout the tissue volume. The solid-matrix protein patch provided a means of controllable energy delivery and yielded consistently strong welds. 1998 Society of Photo-Optical Instrumentation Engineers.

published proceedings

  • J Biomed Opt

altmetric score

  • 3

author list (cited authors)

  • Small, W., Heredia, N. J., Maitland, D. J., Eder, D. C., Celliers, P. M., Da Silva, L. B., London, R. A., & Matthews, D. L.

citation count

  • 5

complete list of authors

  • Small, W||Heredia, NJ||Maitland, DJ||Eder, DC||Celliers, PM||Da Silva, LB||London, RA||Matthews, DL

publication date

  • January 1998