Immunocytochemistry for LHRH neurons in the arcuate nucleus area of the rat: fact or artifact? Academic Article

abstract

• Antisera to luteinizing hormone-releasing hormone (LHRH) generated against hapten-conjugates in which BSA (bovine serum albumin) is used as a carrier can contain both anti-LHRH and anti-BSA antibodies. Such antisera can cause the false-positive staining of neuronal cell bodies and other elements of the medial basal hypothalamus owing to the presence of anti-BSA, which cross-reacts with albuminoid substances within these cells. Differential blocking experiments using either BSA or LHRH or both BSA and LHRH as immunoabsorbents demonstrated that recent reports of LHRH-containing neurons in the medial basal hypothalamus of the rat have arisen from false-positive results due to the presence of anti-BSA within the anti-LHRH antisera used. Immunocytochemical evidence of LHRH-containing cell bodies in the medial basal hypothalamus (especially within the arcuate nucleus) of the rat remains unproven.

authors

• Dees, William

published proceedings

• J Histochem Cytochem

author list (cited authors)

• Kozlowski, G. P., & Dees, W. L.

citation count

• 28

complete list of authors

• Kozlowski, GP||Dees, WL

publication date

• January 1984

publisher

• SAGE Publications  Publisher

published in

• Journal of Histochemistry and Cytochemistry: imaging the spectrum of cell biology  Journal

keywords

• Animals
• Arcuate Nucleus Of Hypothalamus
• Rats

PubMed Central ID

• 6690601

Digital Object Identifier (DOI)

• 10.1177/32.1.6690601

start page

• 83

end page

• 91

volume

• 32

issue

• 1

URL

• http://dx.doi.org/10.1177/32.1.6690601