DNA fingerprinting in horses using a simple (TG)n probe and its application to population comparisons.

abstract

• A synthetic polynucleotide (TG)n was hybridized to equine DNA digested with HinfI and hypervariable hybridization patterns were obtained. Mendelian inheritance of these DNA fingerprinting patterns was confirmed by pedigree analysis. Estimates of the probabilities of identical band patterns in unrelated individuals of different breeds (Swedish Trotters, North Swedish Trotters, Thoroughbreds and Arabians) were in the range 1 x 10(-4) - 7 x 10(-6). The variability derived with the (TG)n probe in horses was higher than what we obtained with several other commonly used probes for DNA fingerprinting. Individuals within breeds tended to be more similar to each other with regard to DNA fingerprint pattern than to individuals of other breeds. Moreover, a parsimony analysis made on the basis of the hybridization patterns gave clustering of individuals within breeds. The possibility of using hypervariable probes for the identification of breed-specific characters is discussed.

authors

• Andersson, Leif

published proceedings

• Anim Genet

altmetric score

• 3

author list (cited authors)

• Ellegren, H., Andersson, L., Johansson, M., & Sandberg, K.

citation count

• 21

complete list of authors

• Ellegren, H||Andersson, L||Johansson, M||Sandberg, K

publication date

• January 1992

publisher

• Wiley  Publisher

published in

• Animal Genetics  Journal

keywords

• Animals
• Biological Evolution
• Breeding
• DNA Fingerprinting
• DNA Probes
• Genetics, Population
• Guanine Nucleotides
• Horses
• Nucleic Acid Hybridization
• Polymorphism, Genetic
• Polynucleotides
• Thymine Nucleotides

PubMed Central ID

• 1570891

Digital Object Identifier (DOI)

• 10.1111/j.1365-2052.1992.tb00012.x

start page

• 1

end page

• 9

volume

• 23

issue

• 1

URL

• http://dx.doi.org/10.1111/j.1365-2052.1992.tb00012.x