Genotyping bovine milk proteins using allele discrimination by primer length and automated DNA sizing technology Academic Article uri icon

abstract

  • A method for genotyping kappa-casein (A, B, E), beta-casein (A1, A2, A3, A5, B) and beta-lactoglobulin (A, B) simultaneously by the use of allele discrimination by primer length combined with automated detection of fragments with a sequencing instrument is described. Seven different mutations within the milk protein genes were analysed in order to distinguish between the alleles examined. The samples were amplified in two separate multiplex polymerase chain reactions (PCRs), which were then pooled and separated according to size in a single lane on the gel. By using stringent PCR conditions, we have been able to achieve allele-specific amplifications and minimize amplification of mis-matched primer for all seven mutations.

altmetric score

  • 3

author list (cited authors)

  • Lindersson, M., Lundn, A., & Andersson, L.

citation count

  • 14

complete list of authors

  • Lindersson, M||Lundén, A||Andersson, L

publication date

  • April 1995

publisher