Expansion of the pig comparative map by expressed sequence tags (EST) mapping. Academic Article

abstract

• We have used a PCR-based approach for the genetical and physical mapping of 34 transcripts isolated from a porcine small intestine cDNA library. All but one gene were regionally localized by using a somatic pig-rodent cell hybrid panel, and 12 genes were mapped by linkage analysis of single-stranded conformational polymorphisms developed in 3' untranslated regions of transcripts. For 20 of the transcripts, the human homolog has already been mapped. This study thus represents a significant contribution to the pig comparative map. Some important findings were that we could clarify the extent of a previously identified inversion event in a region of conserved synteny between SSC6q and HSA1p, that SSC14q does contain a region homologous to HSA1, a situation not clear from earlier ZOO-FISH studies, and that the homology between SSC17 and HSA20 includes the p-arm of HSA20.

authors

• Andersson, Leif

published proceedings

• Mamm Genome

author list (cited authors)

• Fridolfsson, A. K., Hori, T., Winter, A. K., Fredholm, M., Yerle, M., Robic, A., Andersson, L., & Ellegren, H.

citation count

• 25

complete list of authors

• Fridolfsson, AK||Hori, T||Winterø, AK||Fredholm, M||Yerle, M||Robic, A||Andersson, L||Ellegren, H

publication date

• December 1997

publisher

• Springer Nature  Publisher

published in

• MAMMALIAN GENOME  Journal

keywords

• Animals
• Chromosome Mapping
• Cricetinae
• Cricetulus
• DNA, Complementary
• Gene Expression
• Genetic Markers
• Hybrid Cells
• Mice
• Polymerase Chain Reaction
• Polymorphism, Single-Stranded Conformational
• Swine

PubMed Central ID

• 9383283

Digital Object Identifier (DOI)

• 10.1007/s003359900609

start page

• 907

end page

• 912

volume

• 8

issue

• 12

URL

• http://dx.doi.org/10.1007/s003359900609