Cloning of highly polymorphic microsatellites in the horse. Academic Article

abstract

• We have isolated equine microsatellites by screening a genomic library with (TG)n and (TC)n probes. TG microsatellites were found to be more abundant than TC repeats, with an estimated frequency of one per 100,000bp. Sequence analysis of eight TG-positive clones revealed varying structures of the repeat regions; perfect stretches of TG repeats, imperfect stretches of TG repeats and compound regions of TG and TC repeats. Five loci were analysed by PCR and showed extensive polymorphism; three to seven alleles and heterozygosities of 0.40-0.76 were observed when screening 20-30 unrelated individuals. The high degree of polymorphism, their abundance and the possibility of automating the typing procedure make these loci ideal for standardized paternity testing in the horse. Furthermore, we demonstrate that single hairs can be used as starting material for the PCR analysis.

authors

• Andersson, Leif

published proceedings

• Anim Genet

author list (cited authors)

• Ellegren, H., Johansson, M., Sandberg, K., & Andersson, L.

citation count

• 162

complete list of authors

• Ellegren, H||Johansson, M||Sandberg, K||Andersson, L

publication date

• January 1992

publisher

• Wiley  Publisher

published in

• Animal Genetics  Journal

keywords

• Animals
• Base Sequence
• Cloning, Molecular
• DNA
• DNA, Satellite
• Hair
• Horses
• Molecular Sequence Data
• Polymerase Chain Reaction
• Polymorphism, Genetic

PubMed Central ID

• 1443772

Digital Object Identifier (DOI)

• 10.1111/j.1365-2052.1992.tb00032.x

start page

• 133

end page

• 142

volume

• 23

issue

• 2

URL

• http://dx.doi.org/10.1111/j.1365-2052.1992.tb00032.x