ZBED6 modulates the transcription of myogenic genes in mouse myoblast cells. Academic Article uri icon

abstract

  • ZBED6 is a recently discovered transcription factor, unique to placental mammals, that has evolved from a domesticated DNA transposon. It acts as a repressor at the IGF2 locus. Here we show that ZBED6 acts as a transcriptional modulator in mouse myoblast cells, where more than 700 genes were differentially expressed after Zbed6-silencing. The most significantly enriched GO term was muscle protein and contractile fiber, which was consistent with increased myotube formation. Twenty small nucleolar RNAs all showed increased expression after Zbed6-silencing. The co-localization of histone marks and ZBED6 binding sites and the effect of Zbed6-silencing on distribution of histone marks was evaluated by ChIP-seq analysis. There was a strong association between ZBED6 binding sites and the H3K4me3, H3K4me2 and H3K27ac modifications, which are usually found at active promoters, but no association with the repressive mark H3K27me3. Zbed6-silencing led to increased enrichment of active marks at myogenic genes, in agreement with the RNA-seq findings. We propose that ZBED6 preferentially binds to active promoters and modulates transcriptional activity without recruiting repressive histone modifications.

published proceedings

  • PLoS One

altmetric score

  • 1.5

author list (cited authors)

  • Jiang, L., Wallerman, O., Younis, S., Rubin, C., Gilbert, E. R., Sundstrm, E., ... Andersson, L.

citation count

  • 15

complete list of authors

  • Jiang, Lin||Wallerman, Ola||Younis, Shady||Rubin, Carl-Johan||Gilbert, Elizabeth R||Sundström, Elisabeth||Ghazal, Awaisa||Zhang, Xiaolan||Wang, Li||Mikkelsen, Tarjei S||Andersson, Göran||Andersson, Leif

editor list (cited editors)

  • Mantovani, R.

publication date

  • April 2014