The isolation, separation and preservation of Babesia bigemina. Academic Article

abstract

• Experiments were performed in Colombia to separate Babesia bigemina from contaminating organisms. Babesia bigemina was passaged serially through five splenectomized calves. The first calf was inoculated with blood carrying several different organisms, and subsequent subinoculations were done soon after blood smears from each calf were found to be positive for B. bigemina. Five blood passages were carried out in 6.5 days. Babesia argentina, B. major and A. marginale were eliminated as contaminants of the B. bigemina isolated after four passages. A stabilate of the isolated B. bigemina was established. 1974 E. & S. Livingstone.

authors

• Adams, Leslie

published proceedings

• Trop Anim Health Prod

author list (cited authors)

• Bishop, J. P., Adams, L. G., Thompson, K. C., & Corrier, D. E.

citation count

• 9

complete list of authors

• Bishop, JP||Adams, LG||Thompson, KC||Corrier, DE

publication date

• August 1973

publisher

• Springer Nature  Publisher

keywords

• Animals
• Antiprotozoal Agents
• Babesia
• Babesiosis
• Blood
• Cattle
• Cattle Diseases
• Erythrocytes
• Freezing
• Injections, Intravenous
• Male
• Preservation, Biological
• Splenectomy
• Temperature
• Ticks
• Trypan Blue

PubMed Central ID

• 4802625

Digital Object Identifier (DOI)

• 10.1007/BF02251382

start page

• 141

end page

• 145

volume

• 5

issue

• 3

URL

• http://dx.doi.org/10.1007/bf02251382