Optodynamic simulation of -adrenergic receptor signalling. Academic Article uri icon

abstract

  • Optogenetics has provided a revolutionary approach to dissecting biological phenomena. However, the generation and use of optically active GPCRs in these contexts is limited and it is unclear how well an opsin-chimera GPCR might mimic endogenous receptor activity. Here we show that a chimeric rhodopsin/2 adrenergic receptor (opto-2AR) is similar in dynamics to endogenous 2AR in terms of: cAMP generation, MAP kinase activation and receptor internalization. In addition, we develop and characterize a novel toolset of optically active, functionally selective GPCRs that can bias intracellular signalling cascades towards either G-protein or arrestin-mediated cAMP and MAP kinase pathways. Finally, we show how photoactivation of opto-2AR in vivo modulates neuronal activity and induces anxiety-like behavioural states in both fiber-tethered and wireless, freely moving animals when expressed in brain regions known to contain 2ARs. These new GPCR approaches enhance the utility of optogenetics and allow for discrete spatiotemporal control of GPCR signalling in vitro and in vivo.

published proceedings

  • Nat Commun

author list (cited authors)

  • Siuda, E. R., McCall, J. G., Al-Hasani, R., Shin, G., Il Park, S., Schmidt, M. J., ... Bruchas, M. R.

publication date

  • January 1, 2015 11:11 AM