Preparation of short RNA by in vitro transcription. Academic Article

abstract

• Short RNA oligomers are required in many biochemical and biophysical studies. In vitro transcription using T7 RNA polymerase can produce a large amount of specific RNA sufficient for structural studies in a more economical way than chemical synthesis. Here, we provided protocols optimized for the synthesis of very short RNA of 10-20 nucleotides in length by in vitro transcription. These RNA oligomers can be purified conveniently by gel filtration chromatography. We also described how to study RNA and protein binding by gel filtration chromatography.

authors

• Li, Pingwei

published proceedings

• Methods Mol Biol

author list (cited authors)

• Lu, C., & Li, P.

citation count

• 5

complete list of authors

• Lu, Cheng||Li, Pingwei

publication date

• December 2012

publisher

• Humana Press  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Bacteriophage T7
• Chromatography, Gel
• DNA-Directed RNA Polymerases
• Genetic Techniques
• Humans
• RNA
• Transcription, Genetic

PubMed Central ID

• 23065553

Digital Object Identifier (DOI)

• 10.1007/978-1-62703-113-4_5

start page

• 59

end page

• 68

volume

• 941

URL

• http://dx.doi.org/10.1007/978-1-62703-113-4_5