Counterproductive transcriptional and translational regulation of elongation factor 1-alpha synthesis during early development in sea urchins. Academic Article

abstract

• We have isolated a cDNA clone encoding elongation factor 1-alpha (EF1-alpha) and used probes prepared from this cDNA to measure levels of EF1-alpha transcripts during early development. We also determined the fraction of EF1-alpha transcripts in polysomes during this time period. Following the blastula stage there is a sharp increase in the amount of EF1-alpha mRNA. This pattern of accumulation is similar to other previously described sea urchin mRNAs. However, while the level of EF1-alpha mRNA is increasing, the fraction of EF1-alpha mRNA in polysomes decreases. Thus, there is an apparently counter-productive decrease in efficiency of recruitment into polysomes occurring concurrently with an increase in the overall amount of EF1-alpha mRNA.

authors

• Winemiller, Leslie

published proceedings

• Dev Biol

author list (cited authors)

• Peeler, M. T., Kelso-Winemiller, L., Wu, M. F., Skipper, J. K., & Winkler, M. M.

citation count

• 9

complete list of authors

• Peeler, MT||Kelso-Winemiller, L||Wu, MF||Skipper, JK||Winkler, MM

publication date

• January 1990

publisher

• Elsevier  Publisher

published in

• Developmental Biology  Journal

keywords

• Animals
• Gene Expression Regulation
• Peptide Elongation Factor 1
• Peptide Elongation Factors
• RNA, Messenger
• Ribonucleoproteins
• Ribosomes
• Sea Urchins
• Xenopus

PubMed Central ID

• 2257981

Digital Object Identifier (DOI)

• 10.1016/0012-1606(90)90371-o

start page

• 486

end page

• 488

volume

• 142

issue

• 2

URL

• http://dx.doi.org/10.1016/0012-1606(90)90371-o