Counterproductive transcriptional and translational regulation of elongation factor 1-alpha synthesis during early development in sea urchins. Academic Article uri icon


  • We have isolated a cDNA clone encoding elongation factor 1-alpha (EF1-alpha) and used probes prepared from this cDNA to measure levels of EF1-alpha transcripts during early development. We also determined the fraction of EF1-alpha transcripts in polysomes during this time period. Following the blastula stage there is a sharp increase in the amount of EF1-alpha mRNA. This pattern of accumulation is similar to other previously described sea urchin mRNAs. However, while the level of EF1-alpha mRNA is increasing, the fraction of EF1-alpha mRNA in polysomes decreases. Thus, there is an apparently counter-productive decrease in efficiency of recruitment into polysomes occurring concurrently with an increase in the overall amount of EF1-alpha mRNA.

published proceedings

  • Dev Biol

author list (cited authors)

  • Peeler, M. T., Kelso-Winemiller, L., Wu, M. F., Skipper, J. K., & Winkler, M. M.

citation count

  • 9

complete list of authors

  • Peeler, MT||Kelso-Winemiller, L||Wu, MF||Skipper, JK||Winkler, MM

publication date

  • January 1990