Cell Cycle Modelling for Off-line Dynamic Optimisation of Mammalian Cultures Academic Article

abstract

• Mammalian cell cultures producing high-value biopharmaceuticals are expensive and time-consuming to study due to their exclusive dependence on experimentation. A mathematical model has been developed that describes batch/fed-batch hybridoma suspension cultures under normal and chemically-arrested conditions, which is also used to optimise the fed-batch cultures. The optimised strategy was tested experimentally demonstrating that product concentration was closely predicted though the viable cell concentration was partly underestimated. Overall, the model has assisted in reducing the number of experiments required to determine optimal cell culture conditions. Further work is required to improve the model predictability. 2008 Elsevier B.V. All rights reserved.

authors

• Pistikopoulos, Stratos

published proceedings

• 18TH EUROPEAN SYMPOSIUM ON COMPUTER AIDED PROCESS ENGINEERING

author list (cited authors)

• Lam, C. M., Sriyudthsak, K., Kontoravdi, C., Kothari, K., Park, H., Pistikopoulos, E. N., & Mantalaris, A.

citation count

• 4

publication date

• October 2008

publisher

• Elsevier  Publisher

keywords

• Hybridoma
• Mammalian
• Modelling
• Off-line
• Optimisation

Digital Object Identifier (DOI)

• 10.1016/s1570-7946(08)80023-5

start page

• 109

end page

• 114

volume

• 25

URL

• http://dx.doi.org/10.1016/s1570-7946(08)80023-5