FIS activates glnAp2 in Escherichia coli: role of a DNA bend centered at −55, upstream of the transcription start site Academic Article

abstract

• A binding site for the Escherichia coli nucleoid binding protein FIS (factor for inversion stimulation) was identified upstream of a sigma54-dependent promoter, glnAp2. The binding and bending center of FIS is positioned at -55 with respect to the transcription start site (+1). Binding of FIS at this site activates the transcription of glnAp2 both in vivo and in vitro. Furthermore, we substituted the FIS-mediated DNA bending with other protein (cAMP receptor protein or integration host factor)-mediated DNA bending, without changing the position of the bending center. In vitro transcription assays indicated that all DNA bends centered at -55 activate transcriptional initiation of glnAp2, especially when linear templates were used.

authors

• Nan, Beiyan

published proceedings

• FEMS Microbiol Lett

author list (cited authors)

• Huo, Y., Nan, B., You, C., Tian, Z., Kolb, A., & Wang, Y.

citation count

• 6

complete list of authors

• Huo, Yi-Xin||Nan, Bei-Yan||You, Cong-Hui||Tian, Zhe-Xian||Kolb, Annie||Wang, Yi-Ping

publication date

• April 2006

publisher

• Oxford University Press (OUP)  Publisher

published in

• FEMS Microbiology Letters  Journal

keywords

• Base Sequence
• DNA, Bacterial
• Enhancer Elements, Genetic
• Escherichia Coli
• Escherichia Coli Proteins
• Factor For Inversion Stimulation Protein
• Gene Expression Regulation, Bacterial
• Glutamate-ammonia Ligase
• Molecular Sequence Data
• Nucleic Acid Conformation
• Promoter Regions, Genetic
• Transcription, Genetic

PubMed Central ID

• 16553838

Digital Object Identifier (DOI)

• 10.1111/j.1574-6968.2006.00150.x

start page

• 99

end page

• 105

volume

• 257

issue

• 1

URL

• http://dx.doi.org/10.1111/j.1574-6968.2006.00150.x