FIS activates glnAp2 in Escherichia coli: role of a DNA bend centered at −55, upstream of the transcription start site Academic Article uri icon

abstract

  • A binding site for the Escherichia coli nucleoid binding protein FIS (factor for inversion stimulation) was identified upstream of a sigma54-dependent promoter, glnAp2. The binding and bending center of FIS is positioned at -55 with respect to the transcription start site (+1). Binding of FIS at this site activates the transcription of glnAp2 both in vivo and in vitro. Furthermore, we substituted the FIS-mediated DNA bending with other protein (cAMP receptor protein or integration host factor)-mediated DNA bending, without changing the position of the bending center. In vitro transcription assays indicated that all DNA bends centered at -55 activate transcriptional initiation of glnAp2, especially when linear templates were used.

author list (cited authors)

  • Huo, Y., Nan, B., You, C., Tian, Z., Kolb, A., & Wang, Y.

complete list of authors

  • Huo, Yi-Xin||Nan, Bei-Yan||You, Cong-Hui||Tian, Zhe-Xian||Kolb, Annie||Wang, Yi-Ping

publication date

  • January 1, 2006 11:11 AM