Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci Academic Article uri icon


  • © The Royal Society of Chemistry. The bioconversion of second-generation cellulosic ethanol waste streams into biodiesel via oleaginous bacteria is a novel optimization strategy for biorefineries with substantial potential for rapid development. In this study, one- and two-stage alkali/alkali-peroxide pretreatment waste streams of corn stover were separately implemented as feedstocks in 96 h batch reactor fermentations with wild-type Rhodococcus opacus PD 630, R. opacus DSM 1069, and R. jostii DSM 44719T. Here we show using 31P-NMR, HPAEC-PAD, and SEC analyses, that the more rigorous and chemically-efficient two-stage chemical pretreatment effluent provided higher concentrations of solubilized glucose and lower molecular weight (∼70-300 g mol-1) lignin degradation products thereby enabling improved cellular density, viability, and oleaginicity in each respective strain. The most significant yields were by R. opacus PD 630, which converted 6.2% of organic content with a maximal total lipid production of 1.3 g L-1 and accumulated 42.1% in oils based on cell dry weight after 48 h.

altmetric score

  • 0.25

author list (cited authors)

  • Le, R. K., Wells Jr., T., Das, P., Meng, X., Stoklosa, R. J., Bhalla, A., ... Ragauskas, A. J.

citation count

  • 30

publication date

  • January 2017