-Conglycinin enhances autophagy in porcine enterocytes. Academic Article

abstract

• -Conglycinin (-CG) is well known for inducing intestinal allergies and dysfunction in neonates and young pigs. However, the underlying mechanisms are largely unknown. In this study, to clarify the role of autophagy in -CG-induced cytotoxicity, we investigated the effects of -CG on cell viability and autophagy activity in porcine enterocytes (IPEC-1 cells). The results indicated that the cell viability was decreased with the increasing levels of -CG. -CG treatment enhanced the eGFP-LC3 puncta per cells and LC3-II/LC3-I, and the latter was further increased in IPEC-1 cells cultured with bafilomycin A1. We conclude that -CG enhances autophagy activity in enterocytes.

authors

• Wu, Guoyao

published proceedings

• Amino Acids

altmetric score

• 0.25

author list (cited authors)

• Yi, D., Hou, Y., Mei, H., Wang, L., Hu, C., & Wu, G.

citation count

• 4

complete list of authors

• Yi, Dan||Hou, Yongqing||Mei, Huimin||Wang, Lei||Hu, Chien-An Andy||Wu, Guoyao

publication date

• January 2017

publisher

• Springer Nature  Publisher

published in

• Amino Acids  Journal

keywords

• Allergens
• Animal Feed
• Animals
• Antigens, Plant
• Autophagy
• Cell Line
• Cell Survival
• Enterocytes
• Gene Expression Regulation
• Globulins
• Ipec-1 Cells
• Macrolides
• Microtuble-associated Protein Light Chain 3
• Microtubule-Associated Proteins
• Seed Storage Proteins
• Soybean Proteins
• Soybeans
• Swine
• β-conglycinin

PubMed Central ID

• 27761755

Digital Object Identifier (DOI)

• 10.1007/s00726-016-2352-7

start page

• 203

end page

• 207

volume

• 49

issue

• 1

URL

• http://dx.doi.org/10.1007/s00726-016-2352-7