Differential reactivity of bovine lymphocytes to species of Brucella. Academic Article uri icon


  • The reactivity of bovine lymphocytes to 4 species of Brucella was tested in thymidine-uptake assays, using long-term cultured lymphocytes and freshly obtained blood mononuclear cells. Lymphocytes were taken from cows that had been challenge exposed with a virulent strain of B abortus at midgestation. The cows were classified retrospectively as being naturally resistant or susceptible to brucellosis. Lymphocytes taken from these cows had 3 patterns of reactivity with species of Brucella: pattern 1 was defined by reactivity with 4 species (B abortus, B canis, B suis, and B melitensis); pattern 2 was defined by reactivity with all these species, except B melitensis; pattern 3 was defined by reactivity with B abortus and B canis, but not with B suis or B melitensis. There was a statistically significant correlation between susceptibility to brucellosis and expression of lymphocyte cross-reactivity with B suis (P less than 0.01) and with B melitensis (P less than 0.001).

published proceedings

  • Am J Vet Res

author list (cited authors)

  • Smith, R., Kapatsa, J. C., Sherwood, S. J., Ficht, T. A., Templeton, J. W., & Adams, L. G.

citation count

  • 12

complete list of authors

  • Smith, R||Kapatsa, JC||Sherwood, SJ||Ficht, TA||Templeton, JW||Adams, LG

publication date

  • April 1990