Layer-by-layer assembled smectite-polymer nanocomposite film for rapid fluorometric detection of aflatoxin B-1
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Aflatoxin B 1 (AFB 1) is a potent biological toxin produced by fungi Aspergillus flavus and Aspergillus parasiticus. Current quantification methods for AFB 1 are mostly established on immunoaffinity columns which are both costly and labor intensive. Inspired by smectites' high AFB 1 adsorption capacity and affinity, we report the synthesis of a smectite-polyacrylamide (PAM) nanocomposite film for AFB 1 detection and prove the feasibility of such a film for AFB 1 quantification. A layer-by-layer assembly process was developed to achieve uniform morphology and thickness of the nanocomposite films on flat silicon substrates. Positive correlations between the peak fluorescence intensity of the adsorbed AFB 1 and its concentration in the test solutions were obtained. The smectite-PAM nanocomposite film has shown similar AFB 1 adsorption capabilities as the smectite, while exhibiting excellent structural stability in aqueous solutions. Our results suggest the feasibility of using the smectite-PAM nanocomposite film as a simple biosensor to achieve rapid fluorometric quantification of AFB 1.