GREENHOUSE SCREENING OF CITRUS ROOTSTOCKS FOR TOLERANCE TO BICARBONATE-INDUCED IRON CHLOROSIS
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abstract
Eighteen citrus rootstock seedling lines were tested for their tolerance to Fe chlorosis using sand culture. Potassium carbonate was used to induce Fe-deficiency chlorosis. Chlorosis was quantified by 1) visual ratings, 2) SPAD-502 chlorophyll meter readings, 3) leaf chlorophyll concentration, 4) leaf active Fe, and 5) leaf total Fe. The first four criteria were well correlated among each other but not with leaf total Fe. Although any of the first four measurements could be used to quantify chlorosis, visual ratings and SPAD-502 readings were more convenient. The rootstock that have been reported to be tolerant or very susceptible to Fe chlorosis in calcareous soils were rated similarly for tolerance to bicarbonate-induced Fe chlorosis. Nontrifoliate types such as Texas sour orange (C. aurantium L.), Cleopatra mandarin (C. reticulata Blanco), Vangasay lemon (C. limon Burro.), and Ridge pineapple x Milam 1578-201 (C. sinensis L. Osbeck x C. jambhiri) were tolerant to moderately tolerant. Although most of the trifoliate hybrids tested were moderately susceptible to very susceptible, Smooth Seville x Argentine trifoliate {[C. grands (L.) Osbeck x C. aurantium] x Poncirus trifoliata (L.) Raf.} and F-81-12 citrange (C. sinensis x P. trifoliata) exhibited relatively high tolerance to lime-induced Fe chlorosis.
